Complementation analysis of measles virus temperature-sensitive mutants
نویسندگان
چکیده
منابع مشابه
Complementation analysis of measles virus temperature-sensitive mutants.
Two sets of independently isolated measles virus temperature-sensitive mutants were quantitatively tested for complementation. Analysis of the nine possible combinations of representative mutants indicated that only one pair of mutants is noncomplementing. Thus, the measles virus mutants studied to date define five complementation groups.
متن کاملTemperature-Sensitive Mutants of Measles Virus
were isolated after mutagenesis by 5-azacytidine, 5-fluorouracil, or proflavine. The restricted replication of all mutants at 39 C was blocked subsequent to cell penetration and could not be attributed to heat inactivation of virus infectivity. Complementation analysis was made possible through the use of poly-L-ornithine. The members of one complementation group exhibited wild-type RNA synthes...
متن کامل[Isolation of temperature-sensitive mutants of measles virus].
Measles virus differs from other members of paramyxoviruses in its prolonged growth cycle1), its degree of involvement of the cell nucleus2), its lack of neuraminidase3) and the sedimentability of its RNA4). We have undertaken a study of mutants of this virus in the hope that the study might circumvent some of the problems encountered with temperaturesensitive (ts) mutants of other paramyxoviru...
متن کاملIntragenic Complementation among Temperature Sensitive Mutants of Bacteriophage T4d.
gene. in phage, may be defined as the hereditary information determining A the primary structure of a polypeptide chain. In the absence of information concerning mutational alterations of specific proteins, the operational definition of a gene is usually derived from complementation tests. Cells containing two functioning genomes with different recessive mutations in the trans configuration are...
متن کاملAntigenic phenotypes and complementation groups of temperature-sensitive mutants of simian virus 40.
The antigenic phenotypes of several temperature-sensitive mutants of simian virus 40 were determined by an immunofluorescence microtechnique that allowed a very high degree of internal control for the conditions of virus infection and antigenic staining. The tumor (T), U, capsid protein (C), and virion (V) antigens were investigated. Productive infection in monkey cells and abortive infection i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1977
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.21.1.439-441.1977